River Ganga is one of longest (more than 2500 sq kms long) rivers originating in the Himalayan glaciers and interconnects with River Yamuna, several tributaries, River Teesta, and River Brahmaputra to finally drain into the Bay of Bengal. In socio-cultural conceptions and Hindu religious literature its also a divine goddess who liberates and gives salvation to humans. She is pictured as a Goddess sitting on a crocodile and her idols capture the multi-species element in human connection with the river and its distinctive flora fauna. Several national parks, wildlife sanctuaries and biodiversity hotspots are located on the banks of sacred landscape of River Ganga in India (11 states) and neighbouring Bangladesh. The religious sanctity of the holy river interpenetrates with secular aspects of wildlife and nature conservation to create a multispecies protective ring and ensure politico-economic pressures of livelihood (450 Million persons) and pressures of unplanned urbanization in one of the most densely populated parts of India do not completely ravage the river although several national plans have been funded to clean holy river Ganga. Based on muti-sited fieldwork, I combine secondary and historical literature on Project Tiger to decode the deterioration in the sacred landscape of River Ganga. A multi-species approach (divine, human and non-human) enables us to understand the complexities involved in understanding nature of anthropogenic impact on landscape and the changing socio-cultural perceptions of River Ganga in South Asia.