Using museums as the medium, this paper attempts to express different forms of cross-cultural identity, in order to protect and pass on human cultural heritage as a carrier of multiculturalism, and promote the dialogue between anthropology and the museum field. Qinghai Tibetan Medicine Culture Museum, as a non-profit permanent institution of social service, studies, collects, protects and displays material and intangible heritage. Museums are open to the public, accessible and inclusive, and promote diversity and sustainability. At the same time, as the world's only comprehensive national first-class museum with the theme of Tibetan medicine, it shows the long history and profound theoretical system of Tibetan medicine from different angles. This paper discusses how Qinghai Museum of Tibetan Culture can transform potential collection advantages into cultural dissemination advantages, how to combine the inheritance of medical code with cultural integration, dig deep into the hidden exhibition themes, and establish a good cultural exchange and cultural cognition platform, aiming at giving full play to the advantages of museum display and publicity, and strengthening the publicity of Tibetan medicine research results and treatment techniques. Through the above discussion, this paper explores the subject construction of museum anthropology.